Abstract
Our laboratory tested water samples used for cooling low-acid canned foods at a canning facility under investigation by the U.S. Food and Drug Administration. We used an enzyme-linked immunosorbent assay with digoxigenin-labeled antibodies (DIG-ELISA) and real-time PCR as screening methods and confirmed the presence of neurotoxin-producing Clostridium botulinum in the samples by mouse bioassay.
MeSH terms
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Animals
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Bacteriological Techniques / methods
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Botulinum Toxins / biosynthesis
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Botulism / diagnosis
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Botulism / pathology
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Clostridium botulinum / isolation & purification*
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Disease Models, Animal
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Enzyme-Linked Immunosorbent Assay / methods
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Food Industry / methods*
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Food, Preserved*
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Mass Screening / methods
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Mice
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Polymerase Chain Reaction / methods
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United States
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Water Microbiology*