[Isolation PQQ biosynthesis gene cluster from Gluconobacter oxydans based on sorbose-dehydrogenase activity]

Shuying Gao; Xionghua Xiong; Jianhua Wang; Weicai Zhang

[Isolation PQQ biosynthesis gene cluster from Gluconobacter oxydans based on sorbose-dehydrogenase activity]

Wei Sheng Wu Xue Bao. 2010 Aug;50(8):1104-8.

[Article in Chinese]

Authors

Shuying Gao¹, Xionghua Xiong, Jianhua Wang, Weicai Zhang

Affiliation

¹ Beijing Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China. [email protected]

PMID: 20931881

Abstract

Objective: To isolate PQQ biosynthesis gene cluster from Gluconobacter oxydans H24 based on sorbose-dehydrogenase activity.

Methods: A library of Gluconobacter oxydans H24 genomic DNA was constructed with host strains Escherichia coli JM109s, which was integrated of sdh gene at the ptsG site on the chromosome of JM109. By detecting sorbose-dehydrogenase activity, clone of PQQ biosynthesis was isolated and subcloned.

Results: A positive clone was isolated from Gluconobacter oxydans H24 genomic DNA library. Within the 5,400-base-pair DNA fragment five reading frames are presented, corresponding to five of the pqq genes (pqqABCDE). The nucleotide and amino acid sequence showed highly homology to pqq genes of other bacteria.

Conclusion: The pqqABCDE gene cluster was successfully isolated from Gluconobacter oxydans H24 by sorbose dehydrogenase activity.

Publication types

English Abstract
Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Carbohydrate Dehydrogenases / metabolism*
Gluconobacter oxydans / genetics*
Molecular Sequence Data
Multigene Family*
Open Reading Frames
PQQ Cofactor / biosynthesis*

Substances

PQQ Cofactor
Carbohydrate Dehydrogenases
sorbose dehydrogenase