Abstract
Suppressors of cytokine signalling (SOCS) proteins regulate signal transduction, but their role in responses to chemokines remains poorly understood. We report that cells expressing SOCS1 and 3 exhibit enhanced adhesion and reduced migration towards the chemokine CCL11. Focal adhesion kinase (FAK) and the GTPase RhoA, control cell adhesion and migration and we show the presence of SOCS1 or 3 regulates expression and tyrosine phosphorylation of FAK, while also enhancing activation of RhoA. Our novel findings suggest that SOCS1 and 3 may control chemotaxis and adhesion by significantly enhancing both FAK and RhoA activity, thus localizing immune cells to the site of allergic inflammation.
Copyright © 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Adhesion / drug effects
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Cell Line
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Cell Movement / drug effects*
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Chemokine CCL11 / pharmacology*
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Chemotaxis / drug effects
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Enzyme Activation / drug effects
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Focal Adhesion Kinase 1 / chemistry
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Focal Adhesion Kinase 1 / metabolism
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Gene Expression Regulation, Enzymologic / drug effects
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Humans
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Mice
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Phosphorylation / drug effects
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Suppressor of Cytokine Signaling 1 Protein
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Suppressor of Cytokine Signaling 3 Protein
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Suppressor of Cytokine Signaling Proteins / metabolism*
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Tyrosine / metabolism
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rhoA GTP-Binding Protein / metabolism
Substances
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Chemokine CCL11
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Socs1 protein, mouse
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Socs3 protein, mouse
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Suppressor of Cytokine Signaling 1 Protein
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Suppressor of Cytokine Signaling 3 Protein
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Suppressor of Cytokine Signaling Proteins
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Tyrosine
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Focal Adhesion Kinase 1
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Ptk2 protein, mouse
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rhoA GTP-Binding Protein