Objectives: To study Giardia duodenalis assemblages circulating in the health areas of two hospitals in Zaragoza and León (Spain).
Methods: A total of 211 stool samples with Giardia were genotyped by PCR of the tpi gene.
Results: Assemblage B was the most prevalent, both in Zaragoza (84,7%) and León (95,1%). The remaining isolates were identified as AII+B.
Conclusions: We detected the spread of G. duodenalis assemblage B in Zaragoza and in León, with an increase in its prevalence in Zaragoza compared to previous studies.
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