Adaptive mutations in the genomes of enterovirus 71 strains following infection of mouse cells expressing human P-selectin glycoprotein ligand-1

J Gen Virol. 2011 Feb;92(Pt 2):287-91. doi: 10.1099/vir.0.022418-0. Epub 2010 Oct 13.

Abstract

We recently identified human P-selectin glycoprotein ligand-1 (PSGL-1) as a functional enterovirus 71 (EV71) receptor and demonstrated PSGL-1-dependent replication for some EV71 strains in human leukocytes. Here, we report that four out of five PSGL-1-binding strains of EV71 replicated poorly in mouse L929 cells stably expressing human PSGL-1 (L-PSGL-1 cells). Therefore, we compared the replication kinetics and entire genomic sequence of five original EV71 strains and the corresponding EV71 variants (EV71-LPS), which were propagated once in L-PSGL-1 cells. Direct sequence comparison of the entire genome of the original EV71 strains and EV71-LPS variants identified several possible adaptive mutations during the course of replication in L-PSGL-1 cells, including a putative determinant of the adaptive phenotype in L-PSGL-1 cells at VP2-149. The results suggest that an adaptive mutation, along with a PSGL-1-binding phenotype, may facilitate efficient PSGL-1-dependent replication of the EV71 strains in L-PSGL-1 cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptation, Physiological
  • Animals
  • Cell Line
  • Enterovirus A, Human / genetics*
  • Enterovirus A, Human / physiology
  • Gene Expression Regulation
  • Genome, Viral
  • Humans
  • Membrane Glycoproteins / genetics*
  • Membrane Glycoproteins / metabolism*
  • Mice
  • Molecular Sequence Data
  • Mutation
  • Virus Replication / genetics*
  • Virus Replication / physiology*

Substances

  • Membrane Glycoproteins
  • P-selectin ligand protein

Associated data

  • GENBANK/AB550332
  • GENBANK/AB550333
  • GENBANK/AB550334
  • GENBANK/AB550335
  • GENBANK/AB550336
  • GENBANK/AB550337
  • GENBANK/AB550338
  • GENBANK/AB550339
  • GENBANK/AB550340
  • GENBANK/AB550341