Site-specific cleavage of the 40S ribosomal subunit reveals eukaryote-specific ribosomal protein S28 in the subunit head

FEBS Lett. 2010 Nov 5;584(21):4396-400. doi: 10.1016/j.febslet.2010.10.013. Epub 2010 Oct 15.

Abstract

After resolving the crystal structure of the prokaryotic ribosome, mapping the proteins in the eukaryotic ribosome is a challenging task. We applied RNase H digestion to split the human 40S ribosomal subunit into head and body parts. Mass spectrometry of the proteins in the 40S subunit head revealed the presence of eukaryote-specific ribosomal protein S28e. Recombinant S28e was capable of specific binding to the 3' major domain of the 18S rRNA (K(a)=8.0±0.5×10(9) M(-1)). We conclude that S28e has a binding site on the 18S rRNA within the 40S subunit head.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Eukaryota / cytology*
  • Eukaryota / metabolism*
  • Humans
  • RNA, Ribosomal, 18S / chemistry
  • RNA, Ribosomal, 18S / metabolism
  • Recombinant Proteins / metabolism
  • Ribonuclease H / metabolism
  • Ribosomal Proteins / metabolism*
  • Ribosome Subunits, Small, Eukaryotic / metabolism*
  • Species Specificity

Substances

  • RNA, Ribosomal, 18S
  • RPS28 protein, human
  • Recombinant Proteins
  • Ribosomal Proteins
  • Ribonuclease H