Abstract
Rubisco activase is a molecular chaperone that modulates the activation state of Rubisco by catalyzing the ATP-dependent removal of tightly-bound inhibitory sugar-phosphates from Rubisco's catalytic sites. This chapter reports methods developed for the purification of native and recombinant Rubisco activase from leaves and bacterial cells, respectively.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Ammonium Sulfate / chemistry
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Animals
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Arabidopsis / enzymology
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Cell Proliferation
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Chemical Fractionation / methods*
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Chemical Precipitation
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Chromatography, Gel
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Chromatography, Ion Exchange
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Escherichia coli / cytology
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Escherichia coli / genetics*
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Gene Expression
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Plant Leaves / enzymology*
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Plant Proteins / biosynthesis
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Plant Proteins / genetics*
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Plant Proteins / isolation & purification*
Substances
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Plant Proteins
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rca protein, plant
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Ammonium Sulfate