Succinylated polylysine as a possible link between an antibody molecule and deferoxamine

Bioconjug Chem. 1990 Jul-Aug;1(4):291-5. doi: 10.1021/bc00004a011.

Abstract

Modification of antibodies with chelating polymers may be helpful for radioimmunoimaging, radioimmunotherapy, and NMR tomography. Succinylated polylysine was activated with carbodiimide/N-hydroxysulfosuccinimide in dimethyl sulfoxide and isolated as a dry solid. Sulfosuccinimide-esterified polymer was used for the two-stage coupling of an amino-containing chelating agent (deferoxamine) to monoclonal R11D10 (IgG) or its Fab fragment. Conjugates were separated from free components by using gel-chromatography and anion-exchange chromatography. Antibody-coupling efficiency and the loss of its immunoreactivity upon modification have been studied for polymers with different deferoxamine content. Specific binding of 67Ga to the corresponding antigen via the conjugate has been demonstrated.

MeSH terms

  • Antibodies, Monoclonal / chemical synthesis
  • Chelating Agents / chemistry
  • Cross-Linking Reagents / chemistry
  • Deferoxamine / chemical synthesis*
  • Gallium Radioisotopes
  • Immunotoxins / chemical synthesis*
  • Polylysine / analogs & derivatives
  • Succinimides / chemical synthesis*

Substances

  • Antibodies, Monoclonal
  • Chelating Agents
  • Cross-Linking Reagents
  • Gallium Radioisotopes
  • Immunotoxins
  • Succinimides
  • Polylysine
  • Deferoxamine