Growing insights into the safety of bacteriocins: the case of enterocin S37

Yanath Belguesmia; Amar Madi; Daniel Sperandio; Annabelle Merieau; Marc Feuilloley; Hervé Prévost; Djamel Drider; Nathalie Connil

doi:10.1016/j.resmic.2010.09.019

Growing insights into the safety of bacteriocins: the case of enterocin S37

Res Microbiol. 2011 Feb-Mar;162(2):159-63. doi: 10.1016/j.resmic.2010.09.019. Epub 2010 Oct 28.

Authors

Yanath Belguesmia¹, Amar Madi, Daniel Sperandio, Annabelle Merieau, Marc Feuilloley, Hervé Prévost, Djamel Drider, Nathalie Connil

Affiliation

¹ ONIRIS, Ecole Nationale Vétérinaire, Agro-Alimentaire et de l'Alimentation de Nantes-Atlantique, Rue de la Géraudière- BP 82225, 44322 Nantes Cedex 3, France.

PMID: 21035542
DOI: 10.1016/j.resmic.2010.09.019

Abstract

Very few studies have been reported on the cytotoxicity and impact of bacteriocins, and especially enterocins, upon eukaryotic cells. In order to gain more information on the safety of bacteriocins, we focused this study on enterocin S37, a bacteriocin produced by Enterococcus faecalis S37. We observed dose-dependent cytotoxicity toward undifferentiated Caco-2/TC7 cells. Moreover, no significant effect on differentiated monolayer Caco-2/TC7 and no apoptotic features were observed when cells were treated with 10 μg/ml of enterocin S37. The results obtained indicate possible safe use of enterocin S37 in the gastrointestinal tract of animals to prevent pathogen invasion and/or infection.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Apoptosis
Bacteriocins / biosynthesis
Bacteriocins / toxicity*
Bridged-Ring Compounds / metabolism
Bridged-Ring Compounds / toxicity
Caco-2 Cells / drug effects
Dose-Response Relationship, Drug
Enterococcus faecalis / metabolism
Epithelial Cells / drug effects*
Gastrointestinal Tract / drug effects
Hemolysis
Humans

Substances

Bacteriocins
Bridged-Ring Compounds
enterocin