Identification of immunodominant antigens of Mycobacterium bovis by expression library immunization

Vet J. 2011 Oct;190(1):181-3. doi: 10.1016/j.tvjl.2010.09.021. Epub 2010 Oct 29.

Abstract

This study combines two methodologies - vector expression of a genomic library and proteomics - to identify immunogenic proteins of Mycobacterium bovis. Immunization of BALB/c mice with a plasmid DNA pool from the library, containing approximately 8000 clones, induced a humoral response that facilitated the detection of 12 antigenic proteins by Western blotting. Two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and mass spectrometry identified four proteins (Cpn60-1, HSP70, EF-Tu, and AdoHcyase). Such genomic immunization offers the possibility of in vivo screening of potential candidate M. bovis antigens.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Bacterial / genetics*
  • Antigens, Bacterial / immunology
  • Blotting, Western / veterinary
  • Cattle
  • Cloning, Molecular
  • DNA, Bacterial / genetics*
  • Electrophoresis, Polyacrylamide Gel / veterinary
  • Genomic Library*
  • Mass Spectrometry / veterinary
  • Mice
  • Mice, Inbred BALB C
  • Mycobacterium bovis / genetics*
  • Mycobacterium bovis / immunology
  • Proteomics / methods*
  • Tuberculosis, Bovine / diagnosis*
  • Tuberculosis, Bovine / genetics
  • Tuberculosis, Bovine / immunology

Substances

  • Antigens, Bacterial
  • DNA, Bacterial