Pitfalls and technical aspects during the research of intestinal anastomotic healing in rats

Eur Surg Res. 2010;45(3-4):314-20. doi: 10.1159/000320768. Epub 2010 Oct 29.

Abstract

Background: Fundamental experimental research into intestinal anastomotic healing in rodent models will gain increasing interest in the future.

Methods: The aim of this study was to describe our 5-year experience with a standardized experimental setup of small and large bowel anastomoses in a rodent model and present a basic set of assessment tools investigating anastomotic healing. Anastomotic technique, perioperative complications such as anastomotic insufficiency (AI) and obstructive ileus were in the focus.

Results: During different studies with varying study patterns, 167 rat small bowel anastomoses and 120 colonic anastomoses were performed. Overall mortality was 3.6% in small bowel and 2.5% in colonic anastomoses, AI occurred in 2.9 and 4%, respectively. A postoperative obstructive ileus was seen in 3/167 small bowel anastomoses and none in the colonic group.

Conclusion: When performing experimental intestinal anastomoses in a standardized operative setting and critically considering special perioperative issues, the incidence of relevant complications can be maintained at an adequately low level.

Publication types

  • Evaluation Study

MeSH terms

  • Anastomosis, Surgical / adverse effects
  • Anastomosis, Surgical / methods*
  • Animals
  • Colon / pathology
  • Colon / physiology
  • Colon / surgery
  • Hydroxyproline / metabolism
  • Ileum / pathology
  • Ileum / physiology
  • Ileum / surgery
  • Ileus / etiology
  • Intestines / pathology
  • Intestines / physiology*
  • Intestines / surgery*
  • Male
  • Models, Animal
  • Postoperative Complications / etiology
  • Rats
  • Rats, Wistar
  • Wound Healing / physiology*

Substances

  • Hydroxyproline