Cloning, expression, purification, crystallization and preliminary crystallographic analysis of Rv1698, an outer membrane channel protein from Mycobacterium tuberculosis

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Nov 1;66(Pt 11):1525-7. doi: 10.1107/S1744309110037644. Epub 2010 Oct 29.

Abstract

Rv1698 has been reported to be an important outer membrane channel protein of Mycobacterium tuberculosis with unknown function. Recombinant Rv1698 overexpressed in Escherichia coli was purified in detergent solution and crystallized at 295 K using the sitting-drop vapour-diffusion method with ammonium sulfate as a precipitant. The crystals of Rv1698 diffracted to 2.5 Å resolution and belonged to the orthorhombic space group P422, with unit-cell parameters a = b = 122.0, c = 88.9 Å.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Outer Membrane Proteins / chemistry*
  • Bacterial Outer Membrane Proteins / genetics
  • Bacterial Outer Membrane Proteins / isolation & purification
  • Cloning, Molecular
  • Crystallization
  • Crystallography, X-Ray
  • Gene Expression
  • Mycobacterium tuberculosis / chemistry*

Substances

  • Bacterial Outer Membrane Proteins