[Performances of the assay MTBDRplus(®) in the surveillance of rifampicin resistance in Mycobacterium tuberculosis]

M Fabre; Y Hauck; C Pourcel; G Vergnaud; R Vong; C Soler

doi:10.1016/j.patbio.2010.07.006

[Performances of the assay MTBDRplus(®) in the surveillance of rifampicin resistance in Mycobacterium tuberculosis]

Pathol Biol (Paris). 2011 Apr;59(2):94-6. doi: 10.1016/j.patbio.2010.07.006. Epub 2010 Nov 2.

[Article in French]

Authors

M Fabre¹, Y Hauck, C Pourcel, G Vergnaud, R Vong, C Soler

Affiliation

¹ Service de biologie médicale, HIA Percy, 101, avenue H.-Barbusse, 92141 Clamart, France.

PMID: 21050678
DOI: 10.1016/j.patbio.2010.07.006

Abstract

The purpose of the survey was the routine assessment of the MTBDRplus(®) kit performance in the determination and characterization of Mycobacterium tuberculosis resistance to rifampicin. The survey was carried out on a collection of 144 strains (126 of which were resistant to rifampicin) isolated on patients from 15 countries. Sensitivity to antituberculosis drugs was determined by a liquid culture system and the reference method was the amplification and sequencing of a target region of the rpoB gene whose mutations are responsible for rifampicin resistance (codons 507 to 533). The assessed kit was based on a reverse hybridization technique using eight overlapping probes covering the target region and four probes representing the most-frequently observed mutations. The assay performance was found excellent, specificity: 100%, sensitivity: 99.2%; 17 mutations affecting 10 codons were reported, two of which were newly identified.

Publication types

Evaluation Study

MeSH terms

Antitubercular Agents / pharmacology*
Bacterial Proteins / genetics
Codon / genetics
DNA, Bacterial / genetics
DNA-Directed RNA Polymerases
Data Collection
Djibouti / epidemiology
Drug Resistance, Microbial / genetics
Drug Resistance, Multiple, Bacterial / genetics
France / epidemiology
Genotype
Isoniazid / pharmacology
Mutation, Missense
Mycobacterium tuberculosis / drug effects*
Mycobacterium tuberculosis / genetics
Oxidoreductases / genetics
Point Mutation
Polymerase Chain Reaction / methods*
Reagent Kits, Diagnostic*
Rifampin / pharmacology*
Sensitivity and Specificity
Sequence Analysis, DNA
Thailand / epidemiology
Tuberculosis / epidemiology
Tuberculosis / microbiology*
Tuberculosis, Multidrug-Resistant / epidemiology
Tuberculosis, Multidrug-Resistant / microbiology

Substances

Antitubercular Agents
Bacterial Proteins
Codon
DNA, Bacterial
Reagent Kits, Diagnostic
rpoB protein, Mycobacterium tuberculosis
Oxidoreductases
InhA protein, Mycobacterium
DNA-Directed RNA Polymerases
Isoniazid
Rifampin