Analyzing marginal cases in differential shotgun proteomics

Bioinformatics. 2011 Jan 15;27(2):275-6. doi: 10.1093/bioinformatics/btq632. Epub 2010 Nov 11.

Abstract

Summary: We present an approach to statistically pinpoint differentially expressed proteins that have quantitation values near the quantitation threshold and are not identified in all replicates (marginal cases). Our method uses a Bayesian strategy to combine parametric statistics with an empirical distribution built from the reproducibility quality of the technical replicates.

Availability: The software is freely available for academic use at http://pcarvalho.com/patternlab.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bayes Theorem
  • Proteins / metabolism*
  • Proteomics / methods*
  • Software

Substances

  • Proteins