Protein kinase affinity reagents based on a 5-aminoindazole scaffold

Bioorg Med Chem Lett. 2011 Jan 1;21(1):550-4. doi: 10.1016/j.bmcl.2010.10.069. Epub 2010 Oct 21.

Abstract

Affinity reagents that target protein kinases are powerful tools for signal transduction research. Here, we describe a general set of kinase ligands based on a 5-aminoindazole scaffold. This scaffold can readily be derivatized with diverse binding elements and immobilized analogs allow selective enrichment of protein kinases from complex mixtures.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Chromatography, High Pressure Liquid
  • Indazoles / chemical synthesis
  • Indazoles / chemistry*
  • Protein Binding
  • Protein Kinases / chemistry*
  • Protein Kinases / metabolism
  • Signal Transduction
  • Structure-Activity Relationship

Substances

  • Indazoles
  • 5-aminoindazole
  • Protein Kinases