Hippocampal FGF-2 and BDNF overexpression attenuates epileptogenesis-associated neuroinflammation and reduces spontaneous recurrent seizures

J Neuroinflammation. 2010 Nov 18:7:81. doi: 10.1186/1742-2094-7-81.

Abstract

Under certain experimental conditions, neurotrophic factors may reduce epileptogenesis. We have previously reported that local, intrahippocampal supplementation of fibroblast growth factor-2 (FGF-2) and brain-derived neurotrophic factor (BDNF) increases neurogenesis, reduces neuronal loss, and reduces the occurrence of spontaneous seizures in a model of damage-associated epilepsy. Here, we asked if these possibly anti-epileptogenic effects might involve anti-inflammatory mechanisms. Thus, we used a Herpes-based vector to supplement FGF-2 and BDNF in rat hippocampus after pilocarpine-induced status epilepticus that established an epileptogenic lesion. This model causes intense neuroinflammation, especially in the phase that precedes the occurrence of spontaneous seizures. The supplementation of FGF-2 and BDNF attenuated various parameters of inflammation, including astrocytosis, microcytosis and IL-1β expression. The effect appeared to be most prominent on IL-1β, whose expression was almost completely prevented. Further studies will be needed to elucidate the molecular mechanism(s) for these effects, and for that on IL-1β in particular. Nonetheless, the concept that neurotrophic factors affect neuroinflammation in vivo may be highly relevant for the understanding of the epileptogenic process.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain-Derived Neurotrophic Factor / pharmacology*
  • Electroencephalography
  • Fibroblast Growth Factor 2 / pharmacology*
  • Hippocampus* / drug effects
  • Hippocampus* / pathology
  • Hippocampus* / physiopathology
  • Inflammation / pathology*
  • Interleukin-1beta / metabolism
  • Random Allocation
  • Rats
  • Recurrence
  • Seizures / metabolism
  • Seizures / pathology*
  • Seizures / physiopathology
  • Seizures / prevention & control*
  • Status Epilepticus / chemically induced
  • Status Epilepticus / pathology

Substances

  • Brain-Derived Neurotrophic Factor
  • Interleukin-1beta
  • Fibroblast Growth Factor 2