Mirtazapine alters cue-associated methamphetamine seeking in rats

Biol Psychiatry. 2011 Feb 1;69(3):275-81. doi: 10.1016/j.biopsych.2010.09.032. Epub 2010 Nov 20.

Abstract

Background: Methamphetamine (METH) is a potent psychostimulant, repeated use of which can result in a substance abuse disorder. Withdrawn individuals are highly prone to relapse, which may be driven, at least in part, by a hyperresponsivity to METH-associated cues that can prompt METH-seeking. Clinically efficacious pharmacotherapies for METH abuse are critically needed. Mirtazapine (Remeron) is an atypical antidepressant that antagonizes activated norepinephrine(α)₂, histamine₁ serotonin (5-HT)₂(A/C), and 5-HT₃ receptors. This pharmacologic profile prompted our interest in its potential for preventing relapse to METH-taking. This study tested the hypothesis that mirtazapine would attenuate METH-seeking in rats trained to self-administer METH.

Methods: Rats were trained to self-administer METH in a lever-pressing operant task. The effect of mirtazapine on METH-seeking was determined by evaluating lever pressing in the presence of cues previously associated with METH, but in the absence of METH reinforcement. Two paradigms were used: cue reactivity, wherein rats do not undergo extinction training, and a cue-induced reinstatement paradigm after extinction.

Results: Mirtazapine (5.0 mg/kg) pretreatment reduced METH-seeking by ∼ 50% in the first 15 min of cue reactivity and cue-induced reinstatement testing. This mirtazapine dose did not significantly affect motor performance.

Conclusions: This study revealed the overlapping nature of cue reactivity and cue-induced reinstatement procedures and provided preclinical evidence that mirtazapine can attenuate METH-seeking behavior.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antidepressive Agents, Tricyclic / pharmacology*
  • Cues*
  • Drug Interactions
  • Drug-Seeking Behavior / drug effects*
  • Extinction, Psychological
  • Male
  • Methamphetamine / administration & dosage
  • Methamphetamine / antagonists & inhibitors
  • Methamphetamine / pharmacology*
  • Mianserin / analogs & derivatives*
  • Mianserin / pharmacology
  • Mirtazapine
  • Motor Activity / drug effects
  • Rats
  • Rats, Sprague-Dawley
  • Self Administration / methods

Substances

  • Antidepressive Agents, Tricyclic
  • Mianserin
  • Methamphetamine
  • Mirtazapine