Effects of bone marrow stromal cells and umbilical cord blood-derived stromal cells on daunorubicin-resistant residual Jurkat cells

Transplant Proc. 2010 Nov;42(9):3767-72. doi: 10.1016/j.transproceed.2010.08.055.

Abstract

Objective: To observe the effects of the hematopoietic inductive microenvironment (HIM) simulated by stromal cells of different origins on daunorubicin-resistant residual Jurkat cells (Jurkat/DNR cells).

Methods: Jurkat/DNR cells were cultured and identified. Human umbilical cord blood-derived stromal cells (UCBDSCs) and normal human bone marrow stromal cells (BMSCs) were isolated and cocultured with Jurkat/DNR cells. Jurkat/DNR cells were collected after 14 days of coculture and analyzed with regard to cell proliferation and differentiation abilities, apoptosis, drug sensitivity, and MRD1 multidrug resistance gene mRNA expression.

Results: UCBDSC-simulated HIM suppressed proliferation and promoted apoptosis, differentiation, and drug sensitivity of Jurkat/DNR cells more significantly than BMSC-simulated HIM.

Conclusions: Both BMSCs and UCBDSCs reconstruct the leukemic HIM and reverse drug resistance in Jurkat/DNR cells. UCBDSCs reconstruct the leukemic HIM and reverse drug resistance more significantly than BMSCs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B
  • ATP Binding Cassette Transporter, Subfamily B, Member 1 / genetics
  • Apoptosis / drug effects
  • Bone Marrow Cells / physiology*
  • Caspase 3 / metabolism
  • Caspase 7 / metabolism
  • Cell Communication*
  • Cell Cycle / drug effects
  • Cell Differentiation
  • Cell Proliferation / drug effects
  • Coculture Techniques
  • Daunorubicin / pharmacology*
  • Drug Resistance, Neoplasm*
  • Fetal Blood / cytology*
  • Gene Expression Regulation, Leukemic
  • Humans
  • Jurkat Cells
  • Leukemia, T-Cell / genetics
  • Leukemia, T-Cell / metabolism
  • Leukemia, T-Cell / pathology*
  • Proto-Oncogene Proteins c-bcl-2 / genetics
  • RNA, Messenger / metabolism
  • Stromal Cells / physiology*
  • Time Factors
  • Tumor Microenvironment*
  • bcl-2-Associated X Protein / genetics

Substances

  • ABCB1 protein, human
  • ATP Binding Cassette Transporter, Subfamily B
  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • BAX protein, human
  • Proto-Oncogene Proteins c-bcl-2
  • RNA, Messenger
  • bcl-2-Associated X Protein
  • CASP3 protein, human
  • CASP7 protein, human
  • Caspase 3
  • Caspase 7
  • Daunorubicin