[Growth inhibition of transfection of p16 gene to lung adenocarcinoma cell lines Anip973 and AGZY83-a]

B Wang; C Yan; H Gao; Y Wu; Q Wang; C Huang; S Fu; P Li

doi:10.3779/j.issn.1009-3419.2001.06.14

[Growth inhibition of transfection of p16 gene to lung adenocarcinoma cell lines Anip973 and AGZY83-a]

Zhongguo Fei Ai Za Zhi. 2001 Dec 20;4(6):444-7. doi: 10.3779/j.issn.1009-3419.2001.06.14.

[Article in Chinese]

Authors

B Wang¹, C Yan, H Gao, Y Wu, Q Wang, C Huang, S Fu, P Li

Affiliation

¹ Laboratory of Medical Genetics , Harbin Medical University , Harbin, Heilongjiang 150086, P . R. China.

PMID: 21106155
DOI: 10.3779/j.issn.1009-3419.2001.06.14

Abstract

Background: To investigate the suppression effect of tumor suppressor gene p16 on lung adenocarcinoma cell lines.

Methods: p16 expression vector was transfected into high metastasis lung carcinoma cell line, Anip973, and low metastasis lung cancer cell line, AGZY83-a. The suppression effects of p16 gene on cell growth was detected by cell growth curve, cloning efficiency assay, flow cytometry analysis and TUNEL technique.

Results: Overexpression of p16 gene in Anip973 and AGZY83-a could only lead the cells to arrest at G1 phase, but had no effects on cell growth curves, colony for mation rate and apoptosis.

Conclusions: Overexpression of p16 in Anip973 and AGZY83-a can not suppress the growth of the tumor cells.

Publication types

English Abstract