Antisense effect of pyrrolidine-based oxy-peptide nucleic acids in Escherichia coli

Bioorg Med Chem Lett. 2011 Jan 1;21(1):225-7. doi: 10.1016/j.bmcl.2010.11.034. Epub 2010 Nov 11.

Abstract

To investigate the antisense effect of a pyrrolidine-based oxy-peptide nucleic acid (POPNA), we carried out the LacZ reporter assay using a 12-mer trans-l-POPNA conjugated with a cell-penetrating peptide (antisense reagent). The antisense effect of the conjugated POPNA (inhibition of LacZ activity) was comparable to that shown by a Nielsen-type peptide nucleic acid. Furthermore, the conjugated POPNA could switch the LacZ activity over a wide range of ambient temperatures.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Escherichia coli / drug effects*
  • Oligonucleotides, Antisense / chemistry*
  • Oligonucleotides, Antisense / pharmacology
  • Peptide Nucleic Acids / chemical synthesis
  • Peptide Nucleic Acids / chemistry*
  • Peptide Nucleic Acids / pharmacology
  • Pyrrolidines / chemistry*

Substances

  • Oligonucleotides, Antisense
  • Peptide Nucleic Acids
  • Pyrrolidines