Effects of photodynamic exposure on endothelial cells in vitro

E R Andreeva; O O Udartseva; I N Vozovikov; S G Kuzmin; E M Tararak

doi:10.1007/s10517-010-0922-3

Effects of photodynamic exposure on endothelial cells in vitro

Bull Exp Biol Med. 2010 Aug;149(2):262-4. doi: 10.1007/s10517-010-0922-3.

[Article in English, Russian]

Authors

E R Andreeva¹, O O Udartseva, I N Vozovikov, S G Kuzmin, E M Tararak

Affiliation

¹ Institute of Experimental Cardiology, Russian Cardiology Research-and-Production Complex, Federal Agency for High-Technology Medical Care, Moscow, Russia. [email protected]

PMID: 21113506
DOI: 10.1007/s10517-010-0922-3

Abstract

Experiments on cultured human umbilical vein endotheliocytes showed that accumulation of photosensitive dye (aluminum phthalocyanin; PHOTOSENSE) in cells and laser exposure alone were inessential for the viability of endothelial cells. Contrary to this, exposure of the cells which have accumulated aluminum phthalocyanin (an average of 111.1 ng/mg protein) to low-intensity laser (λ=675 nm) led to a dose-dependent reduction of endotheliocyte viability. Hence, cultured endothelial cells can be used for screening of various photosensitizers and preliminary optimization of photodynamic therapy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Survival / drug effects
Endothelial Cells / drug effects*
Endothelial Cells / metabolism
Humans
In Vitro Techniques
Indoles / pharmacokinetics
Indoles / toxicity*
Laser Therapy / methods*
Microscopy, Fluorescence
Organometallic Compounds / pharmacokinetics
Organometallic Compounds / toxicity*
Photochemotherapy / methods*
Tetrazolium Salts
Thiazoles
Umbilical Veins / cytology

Substances

Indoles
Organometallic Compounds
Tetrazolium Salts
Thiazoles
aluminum phthalocyanine
thiazolyl blue