Ex-vivo oocyte retrieval for fertility preservation

Fertil Steril. 2011 Apr;95(5):1787.e15-7. doi: 10.1016/j.fertnstert.2010.11.023. Epub 2010 Dec 4.

Abstract

Objective: To report a novel fertility preservation strategy in a woman with recurrent serous borderline ovarian tumor in the conserved ovary involving ex-vivo retrieval of in vivo matured oocytes and subsequent embryo cryopreservation.

Design: Case report.

Setting: Tertiary infertility care unit.

Patient(s): A 27-year-old woman presented for follow-up visit with a history of borderline serous adenocarcinoma treated conservatively with left oophorectomy and fertility-sparing laparoscopic staging. Ultrasound scan revealed a recurrent disease in the right ovary.

Intervention(s): Ex-vivo retrieval of mature oocytes after ovarian stimulation.

Main outcome measure(s): Fertility preservation.

Result(s): The patient underwent ovarian stimulation followed by a laparotomy and oophorectomy on the day of oocyte retrieval. A puncture of the follicles was performed in the operating theatre with a maximum ischemia time of 14 minutes. Eleven mature oocytes were aspirating, resulting in seven zygotes for cryopreservation.

Conclusion(s): Mature oocytes can be successfully retrieved ex-vivo from the oophorectomy specimen after a controlled ovarian hyperstimulation (COH) protocol. This method provides a possible strategy for fertility preservation in patients with recurrent ovarian cancer without the risk of cancer cells spillage associated with the standard transvaginal oocyte retrieval.

Publication types

  • Case Reports

MeSH terms

  • Adult
  • Cryopreservation / methods*
  • Cystadenocarcinoma, Serous / pathology
  • Cystadenocarcinoma, Serous / surgery
  • Female
  • Fertility / physiology
  • Humans
  • Infertility, Female / prevention & control*
  • Neoplasm Recurrence, Local / surgery
  • Oocyte Retrieval / methods*
  • Oocytes*
  • Ovarian Neoplasms / pathology
  • Ovarian Neoplasms / surgery
  • Ovary / surgery*
  • Ovulation Induction / methods