Up-regulated expression of Notch1 and Jagged1 in human colon adenocarcinoma

J Gao; J Liu; D Fan; H Xu; Y Xiong; Y Wang; W Xu; Y Wang; Y Cheng; G Zheng

doi:10.1016/j.patbio.2010.11.001

Up-regulated expression of Notch1 and Jagged1 in human colon adenocarcinoma

Pathol Biol (Paris). 2011 Dec;59(6):298-302. doi: 10.1016/j.patbio.2010.11.001. Epub 2010 Dec 8.

Authors

J Gao¹, J Liu, D Fan, H Xu, Y Xiong, Y Wang, W Xu, Y Wang, Y Cheng, G Zheng

Affiliation

¹ Department of Digestive Diseases, Wuhan General Hospital of Guangzhou Command PLA, Wuhan, Hubei Province, PR China.

PMID: 21145176
DOI: 10.1016/j.patbio.2010.11.001

Abstract

Deregulated expression of Notch molecules is observed in many malignant tumors, however, the expression of Notch1 and Jagged1 in colon adenocarcinoma is still unknown. This study is to investigate the expression of Notch1 and Jagged1 in human colon adenocarcinoma. Sixty-five human colon adenocarcinoma and 60 adjacent nontumor colon tissue sections were detected by immunohistochemistry. Ten paired fresh surgical human colon adenocarcinoma and adjacent nontumor colon samples were analyzed by Western blot and RT-PCR. Both Notch1 and Jagged1 were expressed in the cytoplasm of neoplastic cells of colon adenocarcinoma tissue. The protein and mRNA levels of both molecules were higher in colon adenocarcinoma than in adjacent nontumor tissue. Moreover, Notch1 was positively correlated with tumor stage. This investigation demonstrates that Notch1 and Jagged1 are up-regulated in human colon adenocarcinoma and suggests that Notch1/Jagged1 signaling might play a role in the development of colon adenocarcinoma.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenocarcinoma / genetics*
Adenocarcinoma / metabolism
Adenocarcinoma / pathology
Adult
Aged
Aged, 80 and over
Calcium-Binding Proteins / genetics*
Calcium-Binding Proteins / metabolism
Calcium-Binding Proteins / physiology
Cohort Studies
Colonic Neoplasms / genetics*
Colonic Neoplasms / metabolism
Colonic Neoplasms / pathology
Female
Gene Expression Regulation, Neoplastic
Humans
Intercellular Signaling Peptides and Proteins / genetics*
Intercellular Signaling Peptides and Proteins / metabolism
Intercellular Signaling Peptides and Proteins / physiology
Jagged-1 Protein
Male
Membrane Proteins / genetics*
Membrane Proteins / metabolism
Membrane Proteins / physiology
Middle Aged
Receptor, Notch1 / genetics*
Receptor, Notch1 / metabolism
Receptor, Notch1 / physiology
Serrate-Jagged Proteins
Up-Regulation / genetics

Substances

Calcium-Binding Proteins
Intercellular Signaling Peptides and Proteins
JAG1 protein, human
Jagged-1 Protein
Membrane Proteins
NOTCH1 protein, human
Receptor, Notch1
Serrate-Jagged Proteins