The expression of peroxisomal protein transcripts increased by retinoic acid during neural differentiation

Maryam Ostadsharif; Kamran Ghaedi; Mohammad Hossein Nasr-Esfahani; Marzieh Mojbafan; Somayeh Tanhaie; Khadijeh Karbalaie; Hossein Baharvand

doi:10.1016/j.diff.2010.11.003

The expression of peroxisomal protein transcripts increased by retinoic acid during neural differentiation

Differentiation. 2011 Feb;81(2):127-32. doi: 10.1016/j.diff.2010.11.003. Epub 2010 Dec 9.

Authors

Maryam Ostadsharif¹, Kamran Ghaedi, Mohammad Hossein Nasr-Esfahani, Marzieh Mojbafan, Somayeh Tanhaie, Khadijeh Karbalaie, Hossein Baharvand

Affiliation

¹ Basic Medical Sciences Department, Islamic Azad University, Khorasgan Branch, Isfahan, Iran.

PMID: 21145646
DOI: 10.1016/j.diff.2010.11.003

Abstract

Peroxisomal matrix protein is mainly expressed in heart, skeletal muscle, and brain tissues. To study the expression of peroxisomal protein (PEP) during neurogenesis, we employed mouse embryonic carcinoma cells (P19) and embryonic stem cells (mESCs) as an in vitro model for neural differentiation by retinoic acid (RA) induction. Expression pattern of PEP was investigated under distinct steps of differentiation by RT-PCR and real-time PCR. The results revealed that expression of PEP transcripts was markedly increased after the RA treatment at embryoid body and neural stages. Therefore, we concluded that PEP might be involved in the early process of neurogenesis, which needs further verification.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Catalase / genetics
Cell Line, Tumor
Embryonal Carcinoma Stem Cells / drug effects
Gene Expression Profiling
Mice
Mice, Inbred C57BL
Neurogenesis / drug effects*
Neurogenesis / genetics
Peroxisomes / drug effects*
Peroxisomes / genetics
Transcription, Genetic / drug effects*
Tretinoin / pharmacology*

Substances

Tretinoin
Catalase