Reduction of immunogenicity of anthrax vaccines subjected to thermal stress, as measured by a toxin neutralization assay

Juan Castelán-Vega; Laura Corvette; Lev Sirota; Juan Arciniega

doi:10.1128/CVI.00267-10

Reduction of immunogenicity of anthrax vaccines subjected to thermal stress, as measured by a toxin neutralization assay

Clin Vaccine Immunol. 2011 Feb;18(2):349-51. doi: 10.1128/CVI.00267-10. Epub 2010 Dec 8.

Authors

Juan Castelán-Vega¹, Laura Corvette, Lev Sirota, Juan Arciniega

Affiliation

¹ Center for Biologics Evaluation and Research, 1401 Rockville Pike, Rockville, Maryland 20852, USA.

Abstract

We report that a toxin neutralization assay (TNA) can detect a decrease in the immunogenicity of anthrax vaccines as a consequence of brief exposure to elevated temperature. This attribute of TNA may help in adopting immunogenicity as a replacement of the current potency test, which involves protection from lethal challenge.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Anthrax Vaccines / immunology*
Anthrax Vaccines / radiation effects*
Antibodies, Bacterial / blood
Antitoxins / blood
Drug Stability
Hot Temperature
Mice
Neutralization Tests

Substances

Anthrax Vaccines
Antibodies, Bacterial
Antitoxins