Enzyme entrapped nanoporous scaffolds formed through flow-induced gelation in a microfluidic filter device for sensitive biosensing of organophosphorus compounds

Lab Chip. 2011 Feb 7;11(3):381-4. doi: 10.1039/c0lc00337a. Epub 2010 Dec 8.

Abstract

A novel and versatile processing method was developed for the formation of gel scaffolds with in situ AChE-AuNPs immobilization for biosensing of organophosphorus compounds. The biosensor designed by our new approach shows high sensitivity, selectivity and reactivation efficiency. This flow-induced immobilization technique opens up new pathways for designing a simple, fast, biocompatible, and cost-effective process for enhanced sensor performance and on-site monitoring of a variety of toxic organophosphorus compounds.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Biocompatible Materials
  • Biosensing Techniques / instrumentation
  • Biosensing Techniques / methods*
  • Environmental Monitoring
  • Enzymes, Immobilized / chemistry*
  • Flow Injection Analysis / methods*
  • Gels*
  • Metal Nanoparticles / chemistry
  • Microfluidic Analytical Techniques / instrumentation*
  • Organophosphorus Compounds / analysis*

Substances

  • Biocompatible Materials
  • Enzymes, Immobilized
  • Gels
  • Organophosphorus Compounds