Abstract
Photoactivated cross-linking of peptides to proteins is a useful strategy for identifying enzyme-substrate and protein-protein interactions in cell lysates as demonstrated by studies on the human hypoxia inducible factor system.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cells, Cultured
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Cross-Linking Reagents / pharmacology*
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Humans
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Hypoxia-Inducible Factor 1, alpha Subunit / chemistry
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Hypoxia-Inducible Factor 1, alpha Subunit / metabolism*
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Kidney / cytology
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Kidney / metabolism
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Molecular Probes
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Peptide Fragments / chemistry
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Peptide Fragments / radiation effects*
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Photochemistry*
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Protein Interaction Mapping
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Spectrometry, Mass, Electrospray Ionization
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Von Hippel-Lindau Tumor Suppressor Protein / chemistry
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Von Hippel-Lindau Tumor Suppressor Protein / metabolism*
Substances
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Cross-Linking Reagents
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HIF1A protein, human
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Hypoxia-Inducible Factor 1, alpha Subunit
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Molecular Probes
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Peptide Fragments
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Von Hippel-Lindau Tumor Suppressor Protein
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VHL protein, human