Abstract
Using adoptive transfer models we determined that an adeno-associated viral vector of serotype 2 (AAV2) induces in mice proliferation of CD8(+) T cells that recognize an epitope within the viral capsid. Proliferation to an endogenous epitope within viral protein (VP)3 could be observed for at least 3 weeks while a foreign epitope placed at multiple copies within VP2 elicited CD8(+) T cell expansion for at least 10 weeks. These data show that capsid antigens of AAV2 degrade slowly over a period of weeks and during this period provide targets to CD8(+) T cells.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Antigen Presentation / immunology
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CD8-Positive T-Lymphocytes / immunology*
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Capsid Proteins / immunology*
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Cells, Cultured
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Dendritic Cells / immunology
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Dependovirus / genetics*
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Epitopes, T-Lymphocyte / chemistry
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Epitopes, T-Lymphocyte / genetics
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Epitopes, T-Lymphocyte / metabolism
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Factor IX / genetics
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Factor IX / metabolism
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Female
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Gene Order
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Gene Transfer Techniques
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Genetic Vectors / genetics*
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Hepatocytes / immunology
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Humans
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Immunity, Cellular
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Lymphocyte Activation / immunology*
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Male
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C57BL
Substances
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Capsid Proteins
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Epitopes, T-Lymphocyte
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Factor IX