[Effect of lipopolysaccharides extracted from Porphyromonas endodontalis on the expression of CD14 in osteoblast]

Shanghai Kou Qiang Yi Xue. 2010 Oct;19(5):521-4.
[Article in Chinese]

Abstract

Purpose: To investigate the effect of lipopolysaccharides(LPS) extracted from Porphyromonas endodontalis(P.e) on the expression of CD14 in osteoblast.

Methods: Under the condition with or without serum, MC3T3-E1 cells were stimulated with 10 μg/mL P.e-LPS for 24 hours, then the expression of CD14 was measured using flow cytometry; the membrane CD14 mRNA was detected at different time point (0, 1, 3, 6, 12, 24 h) by RT-PCR. Statistical analysis was performed using two-sample t test, one-way ANOVA and Dunnett t test with SPSS13.0 software package.

Results: Flow cytometry showed that CD14 protein increased after the stimulation of P.e-LPS for 24 h, while the non-serum group demonstrated more increase; the membrane CD14 mRNA level was up-regulated by 10 μg/mL P.e-LPS at 1 hour, and reached the maximum at 3 h and declined after 6 h.

Conclusions: P.e-LPS can induce the expression of CD14 in osteoblast MC3T3-E1, which indicates that P.e-LPS may play an important role in osteoblast through CD14.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Humans
  • Lipopolysaccharides*
  • Osteoblasts
  • Porphyromonas endodontalis*
  • RNA, Messenger

Substances

  • Lipopolysaccharides
  • RNA, Messenger