Abstract
Pulmonary epithelial cells may be responsible for regulating airway smooth muscle function, in part by release of fatty acid-derived mediators. Incubation of isolated guinea pig tracheal epithelial cells with radiolabeled arachidonic acid (AA) leads to the production of 5- and 15-hydroxyeicosatetraenoic acid (5- and 15-HETE) and smaller amounts of leukotriene (LT) B4 and C4 and 12-hydroxyheptadecatrienoic acid (HHT). Epithelial cells also are able to release linoleic acid (LA) metabolites. Incubation with radiolabeled linoleic acid leads to the formation of 9- and 13-hydroxyoctadecadienoic acid (9- and 13-HODE). The biological significance of these mediators produced by epithelial cells is discussed.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Antithrombins / biosynthesis
-
Arachidonic Acid
-
Arachidonic Acids / metabolism*
-
Carbon Radioisotopes
-
Epithelial Cells
-
Epithelium / metabolism
-
Fatty Acids / metabolism
-
Guinea Pigs
-
Hydroxyeicosatetraenoic Acids / biosynthesis
-
Linoleic Acids / biosynthesis
-
Linoleic Acids / metabolism*
-
Linoleic Acids, Conjugated*
-
Male
-
Perfusion
-
Trachea / metabolism*
Substances
-
Antithrombins
-
Arachidonic Acids
-
Carbon Radioisotopes
-
Fatty Acids
-
Hydroxyeicosatetraenoic Acids
-
Linoleic Acids
-
Linoleic Acids, Conjugated
-
9-hydroxy-10,12-octadecadienoic acid
-
Arachidonic Acid
-
5-hydroxy-6,8,11,14-eicosatetraenoic acid
-
13-hydroxy-9,11-octadecadienoic acid
-
15-hydroxy-5,8,11,13-eicosatetraenoic acid