Photothermal nanoblade for patterned cell membrane cutting

Opt Express. 2010 Oct 25;18(22):23153-60. doi: 10.1364/OE.18.023153.

Abstract

We report a photothermal nanoblade that utilizes a metallic nanostructure to harvest short laser pulse energy and convert it into a highly localized and specifically shaped explosive vapor bubble. Rapid bubble expansion and collapse punctures a lightly-contacting cell membrane via high-speed fluidic flows and induced transient shear stress. The membrane cutting pattern is controlled by the metallic nanostructure configuration, laser pulse polarization, and energy. Highly controllable, sub-micron sized circular hole pairs to half moon-like, or cat-door shaped, membrane cuts were realized in glutaraldehyde treated HeLa cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Absorption
  • Cell Membrane / chemistry*
  • Computer Simulation
  • HeLa Cells
  • Humans
  • Lasers
  • Light*
  • Nanostructures / chemistry*
  • Nanotechnology / methods*
  • Pattern Recognition, Automated / methods*
  • Reproducibility of Results
  • Temperature*