Abstract
Objective:
To develop a method for the detection of RRM1, ERCC1 and BRCA1 gene expression by SYBR real-time fluorescent quantitative PCR in non-small cell lung cancer tissues and peripheral blood.
Methods:
The plasmid standard of RRM1, ERCC1, BRCA1 and β-actin genes was constructed. SYBR real-time PCR was performed, and the standard curve was established. The expressions of RRM1, ERCC1 and BRCA1 mRNA in non-small cell lung cancer tissues and peripheral blood were detected.
Result:
The standard curve presented linearity. The liquate curves of standard gene were all single apex, indicating that a good specificity was obtained.
Conclusion:
The developed SYBR real-time fluorescent quantitative PCR has advantage of convenient operation, low cost, good specificity and high veracity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Actins / genetics
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BRCA1 Protein / blood
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BRCA1 Protein / genetics*
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Carcinoma, Non-Small-Cell Lung / blood
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Carcinoma, Non-Small-Cell Lung / metabolism*
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DNA-Binding Proteins / blood
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DNA-Binding Proteins / genetics*
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Endonucleases / blood
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Endonucleases / genetics*
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Female
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Humans
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Lung Neoplasms / blood
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Lung Neoplasms / metabolism*
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Male
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Middle Aged
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Polymerase Chain Reaction / methods*
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RNA, Messenger / analysis
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RNA, Messenger / blood
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Ribonucleoside Diphosphate Reductase
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Tumor Suppressor Proteins / blood
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Tumor Suppressor Proteins / genetics*
Substances
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Actins
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BRCA1 Protein
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DNA-Binding Proteins
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RNA, Messenger
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Tumor Suppressor Proteins
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RRM1 protein, human
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Ribonucleoside Diphosphate Reductase
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ERCC1 protein, human
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Endonucleases