[Determination of esculeoside A in Lycopersicon esculentum MILL by high performance liquid chromatography with evaporative light scattering detection]

Se Pu. 2010 Sep;28(9):908-11.
[Article in Chinese]

Abstract

A method for the determination of esculeoside A in Lycopersicon esculentum MILL by high performance liquid chromatography (HPLC) with evaporative light scattering detection (ELSD) was established. The results showed that esculeoside A had good linearity in the range of 0.61-3.05 mg with the correlation coefficient of 0. 999 5. The average recoveries were 97.9%-104.8% with the relative standard deviations (RSD) < or = 4.14% (n=5). The method is simple, sensitive and suitable for the analysis of esculeoside A and quality control for the raw material and extract of Lycopersicon esculentum MILL.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, High Pressure Liquid / methods*
  • Light*
  • Sapogenins / analysis*
  • Scattering, Radiation*
  • Solanum lycopersicum / chemistry*

Substances

  • Sapogenins
  • esculeoside A