Development of a URA5 integrative cassette for gene disruption in the Candida guilliermondii ATCC 6260 strain

Yoann Millerioux; Marc Clastre; Andrew J Simkin; Emeline Marais; Andriy A Sibirny; Thierry Noël; Joël Crèche; Nathalie Giglioli-Guivarc'h; Nicolas Papon

doi:10.1016/j.mimet.2010.12.013

Development of a URA5 integrative cassette for gene disruption in the Candida guilliermondii ATCC 6260 strain

J Microbiol Methods. 2011 Feb;84(2):355-8. doi: 10.1016/j.mimet.2010.12.013. Epub 2010 Dec 21.

Authors

Yoann Millerioux¹, Marc Clastre, Andrew J Simkin, Emeline Marais, Andriy A Sibirny, Thierry Noël, Joël Crèche, Nathalie Giglioli-Guivarc'h, Nicolas Papon

Affiliation

¹ Biomolécules et Biotechnologies Végétales, Faculté de Pharmacie, Université François Rabelais, Tours, France.

PMID: 21182877
DOI: 10.1016/j.mimet.2010.12.013

Abstract

We designed an efficient transformation system for Candida guilliermondii based on a ura5 ATCC 6260 derived recipient strain and a URA5 recyclable selection marker. This "URA5 blaster" disruption system represents a powerful tool to study the function of a large pallet of genes in this yeast of clinical and biotechnological interest.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Candida / genetics*
Genes, Fungal
Genetics, Microbial / methods*
Humans
Mutagenesis, Insertional / methods*
Selection, Genetic*
Transformation, Genetic