Abstract
A novel gene, designated mgt-6, containing four splicing variants, was isolated from a gene trap clone library of C3H/10T1/2 cells transfected with retroviral promoterless gene-trap vector, ROSAFARY. The transcript variants were differentially expressed in murine tissues and cell lines and differentially responded to diverse stimuli including TGF-β1 and mitogen-activated protein kinase (MAPK) inhibitors. The mgt-6 gene encoded a protein of 37 or 11 amino acid residuals with cytoplasmic distribution. However, when C3H/10T1/2 cells were treated with 5-azacytidine, the protein translocated into cell nucleus as indicated by fused LacZ or C-terminally tagged EGFP. Our preliminary results suggest that further study on the role of mgt-6 gene in cell transformation and differentiation may be of significance.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alternative Splicing
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Animals
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Cloning, Molecular
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Gene Library
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Imidazoles / pharmacology
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Lac Operon / genetics
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Mesenchymal Stem Cells / metabolism*
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Mice
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Mice, Inbred C3H
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Mitogen-Activated Protein Kinase Kinases / antagonists & inhibitors
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Mitogen-Activated Protein Kinase Kinases / metabolism
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Nuclear Proteins / analysis
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism*
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Pyridines / pharmacology
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Transforming Growth Factor beta1 / antagonists & inhibitors
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Transforming Growth Factor beta1 / metabolism
Substances
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Imidazoles
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Mgt-6 protein, mouse
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Nuclear Proteins
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Pyridines
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Transforming Growth Factor beta1
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enhanced green fluorescent protein
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Green Fluorescent Proteins
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Mitogen-Activated Protein Kinase Kinases
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SB 203580