Crystallization and preliminary X-ray analysis of the vWA domain of human anthrax toxin receptor 1

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Jan 1;67(Pt 1):64-7. doi: 10.1107/S1744309110043770. Epub 2010 Dec 22.

Abstract

The Gram-positive spore-forming bacterium Bacillus anthracis causes anthrax by secreting anthrax toxin, which consists of protective antigen (PA), lethal factor and oedema factor. Binding of PA to receptors triggers the multi-step process of anthrax toxin entry into target cells. Two distinct cellular receptors, ANTXR1 (also known as tumour endothelial marker 8; TEM8) and ANTXR2 (also known as capillary morphogenesis protein 2; CMG2), for anthrax toxin have been identified. Although the crystal structure of the extracellular von Willebrand factor A (vWA) domain of CMG2 has been reported, the difference between the vWA domains of TEM8 and CMG2 remains unclear because there are no structural data for the TEM8 vWA domain. In this report, the TEM8 vWA domain was expressed, purified and crystallized. X-ray diffraction data were collected to 1.8 Å resolution from a single crystal, which belonged to space group P1 with unit-cell parameters a=65.9, b=66.1, c=74.4 Å, α=63.7, β=88.2, γ=59.9°.

MeSH terms

  • Antigens, Bacterial / chemistry
  • Bacterial Toxins / chemistry
  • Crystallization
  • Crystallography, X-Ray
  • Extracellular Matrix Proteins / chemistry*
  • Extracellular Matrix Proteins / genetics
  • Humans
  • Membrane Proteins / chemistry
  • Membrane Proteins / genetics
  • Microfilament Proteins
  • Molecular Sequence Data
  • Neoplasm Proteins / chemistry*
  • Neoplasm Proteins / genetics
  • Protein Conformation*
  • Receptors, Cell Surface / chemistry*
  • Receptors, Cell Surface / genetics
  • Receptors, Peptide

Substances

  • ANTXR1 protein, human
  • ANTXR2 protein, human
  • Antigens, Bacterial
  • Bacterial Toxins
  • Extracellular Matrix Proteins
  • Membrane Proteins
  • Microfilament Proteins
  • Neoplasm Proteins
  • Receptors, Cell Surface
  • Receptors, Peptide
  • VWA1 protein, human
  • anthrax toxin