Ribosomal RNA sequence analysis of Brucella infection misidentified as Ochrobactrum anthropi infection

Rebecca T Horvat; Wissam El Atrouni; Kassem Hammoud; Dana Hawkinson; Scott Cowden

doi:10.1128/JCM.01131-10

Ribosomal RNA sequence analysis of Brucella infection misidentified as Ochrobactrum anthropi infection

J Clin Microbiol. 2011 Mar;49(3):1165-8. doi: 10.1128/JCM.01131-10. Epub 2011 Jan 5.

Authors

Rebecca T Horvat¹, Wissam El Atrouni, Kassem Hammoud, Dana Hawkinson, Scott Cowden

Affiliation

¹ University of Kansas, School of Medicine, Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, Kansas 66160, USA. [email protected]

Abstract

A Brucella isolate was identified from purulent material collected during a hip surgery. Two previous blood cultures from the same patient yielded Ochrobactrum anthropi. After rRNA sequencing, all the isolates were identified as Brucella species and subsequently serotyped as Brucella suis. Misidentification of Brucella species remains a problem with bacterial identification systems.

Publication types

Case Reports

MeSH terms

Blood / microbiology
Brucella suis / classification
Brucella suis / genetics*
Brucella suis / isolation & purification*
Brucellosis / diagnosis*
Brucellosis / microbiology*
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
Female
Hip / pathology
Hip / surgery
Humans
Middle Aged
Ochrobactrum anthropi / classification
Ochrobactrum anthropi / genetics
Ochrobactrum anthropi / isolation & purification
Osteoarthritis / microbiology
Osteoarthritis / surgery
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA

Substances

DNA, Bacterial
DNA, Ribosomal
RNA, Ribosomal, 16S