Generation of antigen-specific cytotoxic T lymphocytes using a leukemic plasmacytoid dendritic cell line as antigen presenting cells

Akie Yamahira; Miwako Narita; Takeshi Nakamura; Norihiro Watanabe; Masami Kaji; Tomoyo Taniguchi; Shigeo Hashimoto; Tatsuo Furukawa; Ken Toba; Yoshifusa Aizawa; Kiyotaka Kuzushima; Masuhiro Takahashi

doi:10.1016/j.leukres.2010.12.003

Generation of antigen-specific cytotoxic T lymphocytes using a leukemic plasmacytoid dendritic cell line as antigen presenting cells

Leuk Res. 2011 Jun;35(6):793-9. doi: 10.1016/j.leukres.2010.12.003. Epub 2011 Jan 8.

Authors

Akie Yamahira¹, Miwako Narita, Takeshi Nakamura, Norihiro Watanabe, Masami Kaji, Tomoyo Taniguchi, Shigeo Hashimoto, Tatsuo Furukawa, Ken Toba, Yoshifusa Aizawa, Kiyotaka Kuzushima, Masuhiro Takahashi

Affiliation

¹ Laboratory of Hematology and Oncology, Graduate School of Health Sciences, Niigata University, Chuou-ku, Niigata, Japan.

PMID: 21216464
DOI: 10.1016/j.leukres.2010.12.003

Abstract

Establishment of a leukemia plasmacytoid dendritic cell line (PMDC05) and intra-lineage transformation from pDCs to mDCs in PMDC05 has been reported. In this paper, we show the applicability of PMDC05 for cellular immunotherapy. By stimulation with LPS, PMDC05 showed enhancement in expression of antigen presentation-associated surface molecules and production of cytokines (IL-12p70 and TNF-α). The antigen presenting ability was markedly increased in PMDC05 stimulated with LPS. By co-culturing of CD8(+) T cells with LPS-stimulated and WT1/CMVpp65 peptide-pulsed PMDC05, WT1/CMVpp65 tetramer(+) cytotoxic T lymphocytes were efficiently generated. These findings reveal the applicability of PMDC05 in cellular immunotherapy for tumor and severe viral infections.

MeSH terms

Antigen Presentation / drug effects
Antigen Presentation / immunology
Antigen-Presenting Cells / immunology*
Antigen-Presenting Cells / metabolism
Antigens / immunology*
Antigens / metabolism
Antigens, Viral / immunology
CD8-Positive T-Lymphocytes / immunology
CD8-Positive T-Lymphocytes / metabolism
Cell Line, Tumor
Cells, Cultured
Coculture Techniques
Dendritic Cells / immunology*
Dendritic Cells / metabolism
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Humans
Immunophenotyping
Immunotherapy, Adoptive / methods
Interleukin-12 / immunology
Interleukin-12 / metabolism
Leukemia / immunology
Leukemia / metabolism
Leukemia / pathology
Lipopolysaccharides / pharmacology
Peptide Fragments / immunology
Phosphoproteins / immunology
T-Lymphocytes, Cytotoxic / immunology*
T-Lymphocytes, Cytotoxic / metabolism
Tumor Necrosis Factor-alpha / immunology
Tumor Necrosis Factor-alpha / metabolism
Viral Matrix Proteins / immunology
WT1 Proteins / immunology

Substances

Antigens
Antigens, Viral
Lipopolysaccharides
Peptide Fragments
Phosphoproteins
Tumor Necrosis Factor-alpha
Viral Matrix Proteins
WT1 (126-134), human
WT1 Proteins
cytomegalovirus matrix protein 65kDa
Interleukin-12