Nanoparticle-mediated delivery of pitavastatin into lungs ameliorates the development and induces regression of monocrotaline-induced pulmonary artery hypertension

Hypertension. 2011 Feb;57(2):343-50. doi: 10.1161/HYPERTENSIONAHA.110.157032. Epub 2011 Jan 10.

Abstract

Pulmonary artery hypertension (PAH) is an intractable disease of the small PAs in which multiple pathogenic factors are involved. Statins are known to mitigate endothelial injury and inhibit vascular remodeling and inflammation, all of which play crucial roles in the pathogenesis of PAH. We tested the hypothesis that nanoparticle (NP)-mediated delivery of pitavastatin into the lungs can be a novel therapeutic approach for the treatment of PAH. Among the marketed statins, pitavastatin was found to have the most potent effects on proliferation of PA smooth muscle cells in vitro. We formulated pitavastatin-NP and found that pitavastatin-NP was more effective than pitavastatin alone in inhibiting cellular proliferation and inflammation in vitro. In a rat model of monocrotaline-induced PAH, a single intratracheal instillation of NP resulted in the delivery of NP into alveolar macrophages and small PAs for up to 14 days after instillation. Intratracheal treatment with pitavastatin-NP, but not with pitavastatin, attenuated the development of PAH and was associated with a reduction of inflammation and PA remodeling. NP-mediated pitavastatin delivery was more effective than systemic administration of pitavastatin in attenuating the development of PAH. Importantly, treatment with pitavastatin-NP 3 weeks after monocrotaline injection induced regression of PAH and improved survival rate. This mode of NP-mediated pitavastatin delivery into the lungs is effective in attenuating the development of PAH and inducing regression of established PAH, suggesting potential clinical significance for developing a new treatment for PAH.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Cell Proliferation / drug effects
  • Cells, Cultured
  • Cytokines / metabolism
  • Dose-Response Relationship, Drug
  • Drug Delivery Systems
  • Fluorescein-5-isothiocyanate / chemistry
  • Fluorescein-5-isothiocyanate / metabolism
  • Humans
  • Hydroxymethylglutaryl-CoA Reductase Inhibitors / administration & dosage
  • Hydroxymethylglutaryl-CoA Reductase Inhibitors / chemistry
  • Hypertension, Pulmonary / chemically induced
  • Hypertension, Pulmonary / drug therapy*
  • Hypertension, Pulmonary / physiopathology
  • Kaplan-Meier Estimate
  • Lung / drug effects*
  • Lung / metabolism
  • Lung / physiopathology
  • Male
  • Microscopy, Fluorescence
  • Monocrotaline
  • Myocytes, Smooth Muscle / cytology
  • Myocytes, Smooth Muscle / drug effects
  • NF-kappa B / metabolism
  • Nanoparticles / chemistry*
  • Nitric Oxide Synthase / metabolism
  • Pulmonary Artery / drug effects
  • Pulmonary Artery / pathology
  • Pulmonary Artery / physiopathology
  • Quinolines / administration & dosage*
  • Quinolines / chemistry
  • Rats
  • Rats, Sprague-Dawley
  • Remission Induction

Substances

  • Cytokines
  • Hydroxymethylglutaryl-CoA Reductase Inhibitors
  • NF-kappa B
  • Quinolines
  • Monocrotaline
  • Nitric Oxide Synthase
  • Fluorescein-5-isothiocyanate
  • pitavastatin