Risks associated with commodity trade: transmission of viral haemorrhagic septicaemia virus (VHSV) to rainbow trout fry from VHSV-carrying tissue-homogenates

Transbound Emerg Dis. 2011 Jun;58(3):224-31. doi: 10.1111/j.1865-1682.2010.01199.x. Epub 2011 Jan 12.

Abstract

Movements of commodity fish present a potential risk of transferring pathogens. Within a study to estimate the risk from imported rainbow trout Oncorhynchus mykiss carcases, fry were exposed to tissue homogenates from market size rainbow trout infected experimentally with viral haemorrhagic septicaemia (VHS) by waterborne exposure to VHS virus (VHSV, isolate of genotype Ia). Tissues were collected from fish that showed clinical signs and from recent mortalities. Homogenates of (i) internal organs, (ii) brain/gills and (iii) muscle tissue were prepared and added to tanks holding the fry. Virus transmission occurred from all tissues tested, causing high mortality of the fry. The results underline the potential risk of introduction of VHSV through the trade of fish products.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Commerce
  • DNA Primers
  • Disease Models, Animal
  • Novirhabdovirus / genetics
  • Novirhabdovirus / isolation & purification*
  • Oncorhynchus mykiss / virology*
  • Rhabdoviridae Infections / mortality
  • Rhabdoviridae Infections / transmission*
  • Risk Assessment
  • Water Microbiology

Substances

  • DNA Primers