Number of stromal precursors in mouse bone marrow and expression of cytokine genes in primary cultures of mouse bone marrow cells during various periods after immunization with S. typhimurium antigens

U F Gorskaya; T A Danilova; M V Mesentzeva; I M Shapoval; A N Narovlyansky; V G Nesterenko

doi:10.1007/s10517-010-0961-9

Number of stromal precursors in mouse bone marrow and expression of cytokine genes in primary cultures of mouse bone marrow cells during various periods after immunization with S. typhimurium antigens

Bull Exp Biol Med. 2010 Oct;149(4):425-7. doi: 10.1007/s10517-010-0961-9.

[Article in English, Russian]

Authors

U F Gorskaya¹, T A Danilova, M V Mesentzeva, I M Shapoval, A N Narovlyansky, V G Nesterenko

Affiliation

¹ Laboratory for Regulation of Immunity, Laboratory for Microbiology of Latent Infections, Laboratory of Cytokines, N F Gamaleya Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences, Moscow, Russia. [email protected]

PMID: 21234434
DOI: 10.1007/s10517-010-0961-9

Abstract

Administration of S. typhimurium microbial mass to mice was followed by a significant increase (by 3-4 times) in the efficiency of cloning and number of stromal precursors in the femoral bone marrow. These parameters were maximum on days 1-3, but returned to normal by the 8th-15th day after immunization. As differentiated from intact animals, the expression of genes for proinflammatory cytokines IL-1β (day 1 after immunization), IL-6 (days 1-3), TNF-α (days 1, 3, and 6), and IFN-α (days 1-3) was detected in bone marrow cultures from immunized mice. The expression of genes for IFN-γ, IL-18, and IFN-α was decreased on days 1, 3, and 6 after immunization of animals, respectively. Gene expression for the anti-inflammatory cytokine IL-4 was observed on day 6 after immunization. Therefore, this system was not characterized by a decrease in the immune response of stromal cells. The stromal component of hemopoietic and lymphoid organs has the vector of influences in response to bacterial antigens. This vector is directed to the stimulation and progression, but not to the suppression of immune reactions. Our results indicate that resident stromal cells play a role in the immune response of the body.

MeSH terms

Animals
Antigens, Bacterial / genetics
Antigens, Bacterial / immunology*
Bone Marrow Cells / cytology*
Bone Marrow Cells / metabolism
Cytokines / biosynthesis
Cytokines / genetics
Immunization
Interferon-gamma / biosynthesis
Interferon-gamma / genetics
Interleukin-1beta / biosynthesis
Interleukin-1beta / genetics
Interleukin-6 / biosynthesis
Interleukin-6 / genetics
Mice
Salmonella typhimurium / immunology*
Stromal Cells / cytology*
Stromal Cells / immunology
Tumor Necrosis Factor-alpha / biosynthesis
Tumor Necrosis Factor-alpha / genetics

Substances

Antigens, Bacterial
Cytokines
Interleukin-1beta
Interleukin-6
Tumor Necrosis Factor-alpha
Interferon-gamma