Abstract
Using evolved pyrrolysyl-tRNA synthetase-tRNA(CUA)(Pyl) pairs, L-phenylalanine, p-iodo-L-phenylalanine and p-bromo-L-phenylalanine have been genetically incorporated into proteins at amber mutation sites in E. coli.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acyl-tRNA Synthetases / chemistry
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Amino Acyl-tRNA Synthetases / genetics
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Amino Acyl-tRNA Synthetases / metabolism*
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Binding Sites / genetics
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Codon, Nonsense / genetics
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Escherichia coli Proteins / chemistry
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Escherichia coli Proteins / genetics*
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Escherichia coli Proteins / metabolism*
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Lysine / analogs & derivatives*
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Lysine / chemistry
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Lysine / genetics
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Lysine / metabolism
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Models, Molecular
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Molecular Structure
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Mutation / genetics
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Phenylalanine / analogs & derivatives*
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Phenylalanine / chemistry
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Phenylalanine / genetics*
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Protein Engineering*
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RNA, Transfer / genetics
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RNA, Transfer / metabolism
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Stereoisomerism
Substances
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Codon, Nonsense
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Escherichia coli Proteins
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Phenylalanine
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RNA, Transfer
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Amino Acyl-tRNA Synthetases
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pyrrolysine
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Lysine