Transcriptional and posttranscriptional regulation of apolipoprotein E, A-I, and A-II gene expression in normal rat liver and during several pathophysiologic states

Biochemistry. 1990 Sep 11;29(36):8430-5. doi: 10.1021/bi00488a033.

Abstract

Assessment of the relative transcription rates and mRNA steady-state levels for apolipoprotein genes E, A-I, and A-II has been performed in normal rat liver, during liver regeneration and following induction of cirrhosis, as well as in rats with inherited analbuminemia associated with hyperlipidemia. Apo E exhibits primarily transcriptional control with an additional component of posttranscriptional control, whereas Apo A-I is controlled primarily at the posttranscriptional level, thus indicating that these genes are regulated independently. The level of control for Apo A-II has not been determined, because of difficulty experienced in measuring the transcription rate of this gene. During liver regeneration, cirrhosis, and analbuminemia, there is a marked increase in the ratio of Apo A-I to Apo E mRNA, resulting from an increase in the Apo A-I mRNA steady-state level and a decrease in Apo E mRNA. These changes are similar in the three pathophysiologic states and seem to occur through a combination of transcriptional and posttranscriptional mechanisms.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Apolipoprotein A-I
  • Apolipoprotein A-II
  • Apolipoproteins A / biosynthesis
  • Apolipoproteins A / genetics*
  • Apolipoproteins E / biosynthesis
  • Apolipoproteins E / genetics*
  • Carbon Tetrachloride Poisoning / metabolism
  • Chemical and Drug Induced Liver Injury / etiology
  • Chemical and Drug Induced Liver Injury / metabolism
  • Gene Expression Regulation
  • Hyperlipidemias / genetics
  • Hyperlipidemias / metabolism
  • Liver / metabolism
  • Liver Cirrhosis, Experimental / chemically induced
  • Liver Cirrhosis, Experimental / metabolism
  • Liver Regeneration
  • RNA Processing, Post-Transcriptional
  • Rats
  • Rats, Mutant Strains / metabolism
  • Transcription, Genetic

Substances

  • Apolipoprotein A-I
  • Apolipoprotein A-II
  • Apolipoproteins A
  • Apolipoproteins E