Ammonium availability affects the ratio of ammonia-oxidizing bacteria to ammonia-oxidizing archaea in simulated creek ecosystems

Appl Environ Microbiol. 2011 Mar;77(5):1896-9. doi: 10.1128/AEM.02879-10. Epub 2011 Jan 14.

Abstract

The ammonia-oxidizing microbial community colonizing clay tiles in flow channels changed in favor of ammonia-oxidizing bacteria during a 12-week incubation period even at originally high ratios of ammonia-oxidizing archaea to ammonia-oxidizing bacteria (AOB). AOB predominance was established more rapidly in flow channels incubated at 350 μM NH(4)(+) than in those incubated at 50 or 20 μM NH(4)(+). Biofilm-associated potential nitrification activity was first detected after 28 days and was positively correlated with bacterial but not archaeal amoA gene copy numbers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Archaea / classification
  • Archaea / genetics
  • Archaea / growth & development*
  • Archaea / metabolism*
  • Bacteria / classification
  • Bacteria / genetics
  • Bacteria / growth & development*
  • Bacteria / metabolism*
  • Biodiversity*
  • Cluster Analysis
  • DNA, Archaeal / chemistry
  • DNA, Archaeal / genetics
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Ecosystem*
  • Environmental Microbiology
  • Genes, rRNA
  • Molecular Sequence Data
  • Oxidation-Reduction
  • Phylogeny
  • Quaternary Ammonium Compounds / metabolism*
  • RNA, Archaeal / genetics
  • RNA, Bacterial / genetics
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Sequence Homology, Nucleic Acid

Substances

  • DNA, Archaeal
  • DNA, Bacterial
  • DNA, Ribosomal
  • Quaternary Ammonium Compounds
  • RNA, Archaeal
  • RNA, Bacterial
  • RNA, Ribosomal, 16S

Associated data

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