Immunodiagnosis of schistosomiasis patients in The Netherlands: comparison of antibody and antigen detection before and after chemotherapy

Trop Med Parasitol. 1990 Sep;41(3):257-61.

Abstract

We investigated in a retrospective study the application of an enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of the schistosome circulating anodic antigen (CAA) to the diagnosis of schistosomiasis in our laboratory. CAA-titres were compared with IgG-titres as determined with an ELISA using soluble egg antigen (SEA) and IgM-titres as determined in an immuno fluorescence assay (IFA) on sections of Rossman's fixed schistosomes. Pre- and post-treatment serum samples of 182 individuals, suspected for schistosomiasis, were used for this study, including 100 control samples. CAA was detected in 20.7% of all patients who were positive in either IFA or SEA-ELISA or both. CAA-titres did not correlate with either IgM-titres or IgG-titres. No significant drop in IgG level was found after specific treatment (Wilcoxon's P = 0.35). In contrast, a significant fall in both IgM-titre (P less than 0.01), and CAA-level (P less than 0.01) was observed. All CAA-titres became negative after chemotherapy.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Helminth / analysis*
  • Antigens, Helminth / analysis*
  • Enzyme-Linked Immunosorbent Assay
  • Feces / parasitology
  • Fluorescent Antibody Technique
  • Follow-Up Studies
  • Humans
  • Immunoglobulin G / analysis
  • Immunoglobulin M / analysis
  • Netherlands
  • Parasite Egg Count
  • Praziquantel / therapeutic use
  • Predictive Value of Tests
  • Retrospective Studies
  • Schistosoma / immunology*
  • Schistosomiasis haematobia / diagnosis*
  • Schistosomiasis haematobia / drug therapy
  • Schistosomiasis mansoni / diagnosis*
  • Schistosomiasis mansoni / drug therapy

Substances

  • Antibodies, Helminth
  • Antigens, Helminth
  • Immunoglobulin G
  • Immunoglobulin M
  • Praziquantel