Objective: To establish an optimum enrichment and purification process of total flavonoids in Schizonepeta tenuifolia by macroporous resins.
Methods: 9 kinds of resins were compared and the best one was chosen. Then the technique condition for separating and purifying total flavonoids from Schizonepeta tenuifolia was studied.
Results: HP-20 was selected for its excellent adsorption and desorption properties, 80% ethanol was found to be the best elution solution. The best result was based on the follows: pH value-6.0, feeding rate-2 BV/h,sample concentration-0.4 g/mL, the ratio of total flavonoids to HP-20 macroporous resin-133. 76 mg/g, elution flowing rate-4 BV/h, eluting agent amount-3 BV. Then the purity reached 74. 31% and the yield was 97.13%.
Conclusion: The total flavonoids of Schizonepeta tenuifolia can be effectively purificated and separated by HP-20 macroporous resin.