Nucleic Acid Amplification of the opa Gene for Detection of Neisseria gonorrhoeae: experience from a diagnostic laboratory

M J Maze; Sheryl Young; Julie Creighton; Trevor Anderson; Anja Werno

doi:10.1128/JCM.01624-10

Nucleic Acid Amplification of the opa Gene for Detection of Neisseria gonorrhoeae: experience from a diagnostic laboratory

J Clin Microbiol. 2011 Mar;49(3):1128-9. doi: 10.1128/JCM.01624-10. Epub 2011 Jan 19.

Authors

M J Maze¹, Sheryl Young, Julie Creighton, Trevor Anderson, Anja Werno

Affiliation

¹ Canterbury Health Laboratories, Christchurch, New Zealand. [email protected]

Abstract

We report results of Neisseria gonorrhoeae nucleic acid amplification testing (NAAT) with the Abbott m2000 PCR at a tertiary laboratory 6 months after its introduction. Of 5,475 specimens tested, 45 samples (0.82%) tested positive for N. gonorrhoeae. Eight were not cultured, but seven tested positive with a porA pseudogene NAAT.

MeSH terms

Adolescent
Adult
Bacterial Outer Membrane Proteins / genetics*
Bacteriological Techniques / methods*
Female
Gonorrhea / diagnosis*
Humans
Male
Neisseria gonorrhoeae / genetics
Neisseria gonorrhoeae / isolation & purification*
Nucleic Acid Amplification Techniques / methods*
Porins / genetics
Sensitivity and Specificity
Young Adult

Substances

Bacterial Outer Membrane Proteins
Porins
porin protein, Neisseria
Opa protein, Neisseria