Abstract
Expression levels of the novel tumor suppressor gene REIC/Dkk-3 are reduced in many human cancers. We have previously showed that an adenovirus vector carrying REIC/Dkk-3 (Ad-REIC) induced apoptosis of cancer cells selectively and exerted bystander antitumor effects via ER stress. We examined possible effects of Ad-REIC in a peritoneal dissemination model of scirrhous gastric carcinoma (SGC). Among various types of gastric cancer, SGC continues to be associated with the worst prognosis due to a high incidence of metastases in the peritoneal cavity. We found that a single intraperitoneal injection of Ad-REIC suppressed tumor dissemination and disease progression. Immunomodulation by Ad-REIC led to recruitment of natural killer cells inside tumor nodules. We conclude that Ad-REIC gene therapy may be a potential tool in combinatorial approaches to achieve curative effects in SGC.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adaptor Proteins, Signal Transducing
-
Adenocarcinoma, Scirrhous / genetics
-
Adenocarcinoma, Scirrhous / pathology
-
Adenocarcinoma, Scirrhous / therapy*
-
Adenoviridae / genetics
-
Animals
-
Apoptosis
-
Blotting, Western
-
Cell Line, Tumor
-
Chemokines
-
Female
-
Genetic Therapy*
-
Genetic Vectors / administration & dosage*
-
Humans
-
Immunoenzyme Techniques
-
Injections, Intraperitoneal
-
Intercellular Signaling Peptides and Proteins / genetics*
-
Mice
-
Mice, Inbred BALB C
-
Mice, Nude
-
Peritoneal Neoplasms / genetics
-
Peritoneal Neoplasms / secondary
-
Peritoneal Neoplasms / therapy*
-
RNA, Messenger / genetics
-
Reverse Transcriptase Polymerase Chain Reaction
-
Stomach Neoplasms / genetics
-
Stomach Neoplasms / pathology
-
Stomach Neoplasms / therapy*
Substances
-
Adaptor Proteins, Signal Transducing
-
Chemokines
-
DKK3 protein, human
-
Intercellular Signaling Peptides and Proteins
-
RNA, Messenger