Treatment of experimental anterior and intermediate uveitis by a dexamethasone intravitreal implant

Invest Ophthalmol Vis Sci. 2011 May 2;52(6):2917-23. doi: 10.1167/iovs.10-5939.

Abstract

Purpose: To assess the efficacy of a dexamethasone (DEX) intravitreal implant in a rabbit model of anterior and intermediate uveitis.

Methods: Experimental anterior and intermediate uveitis was induced by a unilateral intracameral injection of Mycobacterium tuberculosis H37Ra antigen in preimmunized rabbits. Four days after uveitis induction, rabbits received DEX implant or underwent a sham procedure (no implant). Clinical and histopathologic signs of uveitis were assessed for 13 days, and levels of inflammatory markers in the iris/ciliary body were measured after 21 days.

Results: All signs of anterior and intermediate uveitis were reduced by the DEX implant compared with sham procedure. At day 13, mean anterior chamber cell scores ± SD for the DEX implant versus the sham procedure were, respectively, 1.9 ± 1.3 versus 4.0 ± 0.0 (P = 0.04), and mean total histologic inflammatory scores were 3.9 ± 2.5 versus 15.4 ± 6.0 (P = 0.026). Similarly, at day 13, mean vitreous haze severity scores (SD) for the DEX implant versus the sham procedure were, respectively, 0.1 ± 0.2 versus 2.7 ± 1.5 (P = 0.026), and mean vitreous inflammatory cell infiltration scores were 0.0 ± 0.0 versus 1.5 ± 1.3. Treatment with the DEX intravitreal implant also significantly reduced the proinflammatory immune response, as measured by cytokine levels in iris/ciliary body.

Conclusions: A single administration of DEX implant significantly reduced inflammation in an animal model of anterior and intermediate uveitis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Bacterial / toxicity
  • Biomarkers / metabolism
  • Ciliary Body / metabolism
  • Cytokines / metabolism
  • Dexamethasone / administration & dosage*
  • Disease Models, Animal*
  • Drug Implants
  • Glucocorticoids / administration & dosage*
  • Iris / metabolism
  • Mycobacterium tuberculosis / immunology
  • Rabbits
  • Treatment Outcome
  • Tuberculosis, Ocular / diagnosis
  • Tuberculosis, Ocular / drug therapy*
  • Tuberculosis, Ocular / metabolism
  • Tuberculosis, Ocular / microbiology
  • Uveitis, Anterior / diagnosis
  • Uveitis, Anterior / drug therapy*
  • Uveitis, Anterior / metabolism
  • Uveitis, Anterior / microbiology
  • Uveitis, Intermediate / diagnosis
  • Uveitis, Intermediate / drug therapy*
  • Uveitis, Intermediate / metabolism
  • Uveitis, Intermediate / microbiology
  • Vitreous Body

Substances

  • Antigens, Bacterial
  • Biomarkers
  • Cytokines
  • Drug Implants
  • Glucocorticoids
  • Dexamethasone