The aim of this study was to evaluate several chemical pretreatments on the shear bond strength of three dentin bonding agents (DBAs) and to try to correlate dentin morphology with bond strength using scanning electron microscopy (SEM). The DBAs tested were: Gluma Primer in association with Scotchbond DC, Scotchbond DC alone and Clearfil New Bond. Bond strengths of Scotchbond DC and Clearfil New Bond were not significantly modified or reduced by acidic dentin pretreatments. Gluma/Scotchbond adhesion was increased by several treatments but only maleic acid treatment produced shear bond strengths significantly higher than EDTA treatment. SEM evaluation of dentin treatments revealed a wide variety of morphological changes in the dentin surface with partial and complete removal of smear layer by acidic solutions, and only minor modification by amino acid solutions. Only maleic acid was capable of the complete removal of the smear layer and smear plugs coupled with extensive exposure of dentin collagen fibrils.